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Tosoh Bioscience
Tosoh Bioscience (Japan) product portfolio encompasses a comprehensive line of process media and pre-packed HPLC columns for all modes of liquid chromatography and GPC instruments. For over forty years, Tosoh has been a world leader in the analysis and purification of proteins. A thorough understanding of the role played by pore diameter and molecular size in chromatographic separations allows Tosoh to design higher performance resins for size exclusion, ion exchange, hydrophobic interaction, mixed mode and affinity applications.
TSKgel columns for (U)HPLC are used for the analysis and purification of proteins, peptides, biopolymers and low molecular weight compounds. Tosoh provides (U)HPLC columns for many chromatographic modes such as hydrophobic/hydrophilic interaction, ion exchange, reversed phase, and affinity chromatography. TSKgel columns are known for their reliability and suitability for a variety of chromatographic applications.
TSKgel SW series is the leading SEC column series for HPLC- and UHPLC due to its high internal pore volume, low residual adsorption and excellent resolution. TSKgel G3000SWXL column is the industry’s gold standard for HPLC analysis of antibodies. TSKgel UP-SW3000 columns set standards in UHPLC analysis of antibodies. TSKgel SW, SWXL, SuperSW, UltraSW, and UP-SW are silica SEC phases with pore size distributions suited to protein separations. They are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW series columns are ideal for peptides, proteins and nucleic acids using an aqueous buffer as mobile phase. TSKgel SuperSW mAb and TSKgel UP-SW3000 columns are next generation, small particle size columns for the analysis of monoclonal antibodies by HPLC and UHPLC. TSKgel SW series columns are stable from pH 2.5 to 7.5 and can be used in 100 % aqueous conditions.
TSKgel PW series phases are hydrophilic, rigid, spherical, porous methacrylate beads for aqueous SEC. Wide choice of pore sizes for separation ranges up to 2 x 107 Da for linear polymers. TSKgel PW and TSKgel PWXL polymer based stationary phases are designed for SEC of water soluble organic polymers, polysaccharides, DNA, and RNA. They are based on a hydrophilic polymethacrylate matrix. TSKgel PW series columns are stable in broad pH range from pH 2 to 12 and can be used in aqueous mobile phases. SEC separation is based on the difference of apparent molecular size with no additional interaction between the column matrix and the sample molecules.
TSKgel H series columns are recommended for the analysis of organic-soluble polymers and are packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB). This series includes TSKgel HXL, HHR, SuperH, SuperHZ, and SuperMultiporeHZ columns. High temperature GPC columns for use up to 220°C.
Ion Exchange Chromatography (IEC) retains molecules based on ionic interactions. The stationary phase surface displays ionic functional groups that interact with analyte ions of opposite charge. Ion exchange stationary phases are classified as weak or strong ion exchangers. Top-performer for fast, highly efficient biomolecule separation – TSKgel STAT. First choice for very large proteins – TSKgel BioAssist. Small molecular weight molecules – TSKgel 2SW/3SW.
Hydrophobic Interaction Chromatography (HIC) is used primarily for the separation of non-polar and hydrophobic compounds under non-denaturing conditions. HIC is based on non-polar interactions that are induced by high salt mobile phases. Stationary phases are similar to reversed phase chromatography (RPC) but the density of functional groups is lower.
Hydrophilic Interaction Liquid Chromatography (HILIC) is used primarily for the separation of polar and hydrophilic compounds. HILIC stationary phases are polar, similar to normal phase chromatography (NPC), but mobile phases are similar to reversed phase chromatography (RPC). TSKgel Amide-80 and TSKgel NH2-100.
Reversed phase chromatography (RPC) retains molecules based on their hydrophobic character on a non-polar stationary phase. In an aqueous, moderately polar solvent the hydrophobic patches of the analyte molecule bind to an immobilized hydrophobic ligand. Top performer for protein separation – TSKgel Protein C4-300. First choice for high throughput analysis – TSKgel ODS-140HTP. Standard phases for small molecules – TSKgel ODS-100V/Z. High pH analysis – TSKgel PW or NPR columns
Toyopearl resins are hydrophilic macroporous methacrylic resins. Their rigid polymeric backbone has better pressure-flow properties than most other stationary phases. Therefore, higher linear velocities can be used to achieve faster purification cycles. The resins are offered in many different pore diameters for size exclusion, ion exchange, hydrophobic interaction, multimodal, and affinity chromatography.
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